This will exclude biomass, exchange and demand reactions as they are unbalanced by definition. It will also fail all reactions where at least one metabolite does not have a formula defined. In steady state, for each metabolite the sum of influx equals the sum of efflux. Hence the net masses of both sides of any model reaction have to be equal. Reactions where at least one metabolite does not have a formula are not considered to be balanced, even though the remaining metabolites participating in the reaction might be. Implementation: For each reaction that isn't a boundary or biomass reaction check if each metabolite has a non-zero elements attribute and if so calculate if the overall element balance of reactants and products is equal to zero.

A total of 37 (4.50%) reactions are mass unbalanced with at least one of the metabolites not having a formula or the overall mass not equal to 0: ALATRS, e_Teichuronic_acid, e_Teichoic_acid, ILETRS, e_Lipoteichoic_acid, ...

A large fraction of model reactions able to carry unlimited flux under default conditions indicates problems with reaction directionality, missing cofactors, incorrectly defined transport reactions and more. Implementation: Without changing the default constraints run flux variability analysis. From the FVA results identify those reactions that carry flux equal to the model's maximal or minimal flux.

A fraction of 17.53% of the non-blocked reactions (in total 64 reactions) can carry unbounded flux in the default model condition. Unbounded reactions may be involved in thermodynamically infeasible cycles: AHSERL2, MTHFCx, R03239, R03238, R03237, ...